Finding a reliable sealing film for 96-well plates is essential for preventing evaporation, cross-contamination, and ensuring consistent results in PCR, qPCR, and ELISA workflows. This guide highlights top sealing films that fit standard 96-well plates, focusing on self-adhesive, transparent options with strong seals. The selections below emphasize ease of use, compatibility with common lab instruments, and predictable performance across temperatures and workflows.
Summary of Selected Products
| Product | Brand | Size | Type | Notable Features |
|---|---|---|---|---|
| PCR Plate Sealing Film – 96 Well | Scienfocus lab | 130 x 80mm | Self-Adhesive | Easy tear opening; strong viscosity; high transparency; leak-proof |
| 100 Sheet PCR Sealing Film – 83x133mm | Feleolibe | 83x133mm | Self-Adhesive | Pack of 100; clear sealing film |
| KIESEV PCR Sealing Film – 96 Well | KIESEV | 130 x 80mm | Self-Adhesive | Transparent; easy tear; high viscosity; not for high heat |
| KIESEV PCR Sealing Film – 96 Well (Transparent & Number) | KIESEV | 130 x 80mm | Self-Adhesive | Transparent; with numbers; easy tear |
| PCR Plate Sealing Film – Optically Clear | Albert Bio | 100 sheets pack | Pressure-Sensitive | Tight seal; DNase/RNase-free; not sticky to skin |
Scienfocus Lab PCR Plate Sealing Film
Product overview: Designed for 96-well PCR plates, this sealing film comes in a pack of 100 sheets with 130 x 80mm dimensions. It features an easy tear opening to simplify handling with gloves and maintains strong viscosity after pressing, forming a tight seal to minimize evaporation. The film is highly transparent, allowing easy sample inspection and identification. It aims to seal plates tightly with minimal leakage, supporting reliable amplification and downstream analyses. The film’s performance is optimized for routine PCR and qPCR workflows where consistent sealing is critical.
Key features and considerations
- Easy tearable opening for quick application without tools
- Strong stickiness to ensure a secure seal
- High transparency to view samples without removing the film
- Designed to minimize leakage and evaporation during thermal cycling
Application notes: Suitable for standard 96-well plates, especially in PCR and qPCR settings. Confirm compatibility with your plate brand and instrument before large-scale use.
100 Sheet PCR Sealing Film – 83x133mm
This product offers 100 sheets of sealing film sized at 83x133mm, packaged by Feleolibe. It is positioned as a straightforward self-adhesive solution for sealing 96-well plates, aiding in evaporation control and sample integrity across temperatures typical in PCR workflows. The packaging of 100 sheets makes it suitable for regular lab use without frequent restocking.
Key features and considerations
- Standard 83x133mm sheet size designed for common 96-well plates
- 100 sheets per package provide long-term supply
- Self-adhesive film for quick, tool-free sealing
Notes: Check your plate dimensions and whether the film requires any trimming for your specific plate model. Suitable for routine molecular biology workflows.
KIESEV Sealing Film – 96 Well (Transparent & Number)
From KIESEV, this 130 x 80mm sealing film is packaged as 100 sheets per pack and is designed for sealing various plates including PCR plates, enzyme plates, deep-well plates, and culture plates. While it provides high transparency and strong viscosity, it is not recommended for high-temperature PCR instrument use. The product emphasizes self-tearing for easy removal and a clean seal, reducing the risk of residual adhesive issues on the plate.
Key features and considerations
- Suitable for multiple plate formats beyond PCR plates
- Self-tearing design for easy handling
- High transparency and strong seal without residual glue
- Not recommended for high-temperature instrument use
Usage guidance: Ideal for standard thermal cycling and routine plate-based assays where temperature requirements are moderate. Verify instrument compatibility to avoid deformation at higher temperatures.
KIESEV Sealing Film – 96 Well (Transparent)
This variant shares the same 130 x 80mm size and a 100-sheet pack, highlighting transparency and easy-to-tear properties. It is described as compatible with a range of plate formats. The product stresses that it should not be used with high-temperature PCR instruments, aligning with safe use practices for many standard molecular assays. A numbered version can aid sample tracking across experiments.
Key features and considerations
- Transparent self-adhesive film for easy sample inspection
- Numbers on the film can assist with sample labeling
- Not suitable for high-temperature instrument use
Practical note: Consider lab workflow needs such as sample labeling and plate brand compatibility when selecting this option.
Albert Bio PCR Plate Sealing Film – Optically Clear
Albert Bio offers a pressure-sensitive, optically clear sealing film pack of 100 sheets. It emphasizes a tight seal to minimize evaporation and condensation, with a formulation free of DNase, RNase, or PCR inhibitors. It is noted as not sticky to skin and gloves, reducing transfer risk during handling. This option is positioned for general PCR, qPCR, and ELISA workflows requiring clear visualization of wells while maintaining sample integrity.
Key features and considerations
- Tight seal to reduce evaporation and condensation
- Free of DNase, RNase, and PCR inhibitors
- Skin/glove-friendly surface without residue
Considerations: Ensure your plate type and thermocycler acceptance align with this film’s specifications. This option is suitable for routine molecular assays where clarity and contamination control are priorities.
Buying Guide: Key Purchase Considerations For 96-Well Plate Sealing Films
- Film dimensions and plate compatibility: Most 96-well formats use 130 x 80mm or 83x133mm sheets. Verify sheet size against your plate brand to ensure full coverage without overhang that could cause wells to unseal.
- Adhesion strength vs. ease of removal: Strong adhesion reduces evaporation and leakage but should still allow clean removal without tearing or leaving adhesive residue on plates or instruments.
- Material compatibility and temperature range: Many films are not suitable for high-temperature PCR instruments. If your workflow includes cycling at high temperatures, choose films explicitly rated for such conditions.
- Transparency and labeling: High transparency benefits visual inspection of wells, while some variants include numbers or markers for tracking samples during experiments.
- Sterility and inhibitors: Films marketed as DNase/RNase-free help minimize contamination risks in sensitive assays. Consider this for qPCR and ELISA workflows.
- Packaging and quantity: Packages range from packs of 50 to 100 sheets or more. Assess your lab usage rate to avoid frequent repurchases while controlling storage space and waste.
- Glove-friendly handling: Easy-open features and tearable edges reduce the risk of tearing or sticking to gloves, improving workflow efficiency in biosafety settings.
- Temperature stability and deformation: Some films deform above certain temperatures. If you use heat blocks or very aggressive cycling, confirm temperature resilience before selection.
- Etching and tracking features: Some films offer printed numbers or marks for easier sample identification across wells and experiments. This helps in complex assay layouts and data traceability.
- Instrument compatibility: Check if your real-time PCR machines or plate readers require specific sealing film properties to avoid interference with optics or internal sensors.
This selection emphasizes a balance between strong, reliable sealing and practical handling for routine molecular biology workflows. When choosing among these options, align the film’s specifications with your plate brand, instrument compatibility, and the intended application (PCR, qPCR, ELISA, or general culture work). Always perform a small pilot test to confirm seal integrity and evaporation control under your exact conditions.